BioPhenoMA Contract Research Services
Quantifying the dynamics of real samples in extremely small amounts while preserving localization information.
Across oncology, neurodegeneration, infectious diseases, and metabolic disorders,
the essence of biological phenomena lies not in static concentrations, but in the dynamic molecular responses triggered by stimuli or treatment conditions.
Leveraging our proprietary TN-cyclon™ technology, BioPhenoMA has launched a mechanism oriented contract research service that provides an integrated workflow:
Pre processing → Fractionation → Ultra trace quantification → Dynamics analysis → Mechanistic interpretation
What researchers truly want to understand is:
“Which proteins are released, when, from where, and in what quantity?”
In other words—the dynamic molecular behavior underlying disease biology.
However, quantifying these dynamics directly in real biological specimens has been fundamentally impossible with conventional technologies.
■ Challenges Researchers Have Faced
■ Limitations of Conventional Technologies
-
Microscopy
Can visualize dynamic behavior,
but cannot quantify population‑level molecular changes.
-
Conventional ELISA
Can handle samples containing structural components,
but cannot detect trace‑level proteins.
-
Digital immunoassays /Electrohemiluminescence(ECL)
Can detect trace amounts,
but rely on homogeneous liquid samples.
When applied to real-specimens containing structures such as cells or exosomes,
localization information is lost.
In short, conventional technologies force a binary choice:
“Preserve localization but sacrifice sensitivity,”
or
“Gain sensitivity but lose localization.”
There has been no method capable of achieving both.
■ The Gap Filled by TN-cyclon™
TN-cyclon™ is the only measurement platform that simultaneously enables:
• Localization preserved analysis (membrane vs. lumen of exosomes)
• Ultra trace quantification (0.1 pg/mL)
• Dynamics assessment
• High throughput processing
It is the world’s first technology that can quantify phenomena previously only visible under a microscope—
and do so using real biological specimens across many samples.
■ Key Features of TN-cyclon™
TN-cyclon™ is an advanced evolution of ELISA, engineered specifically for real specimen dynamics analysis.
• Localization preserved: Membrane side vs. lumen side protein distribution
• Ultra trace sensitivity: Down to 0.1 pg/mL
• Compatible with standard laboratory equipment
• Optimized for high throughput studies
■ Value of BioPhenoMA Contract Research Services
BioPhenoMA is not a simple measurement provider.
We deliver mechanism oriented research support, enabling:
“From sample to mechanism.”
■ Service Components
• Specimen pre processing
(Blood, urine, saliva, throat swabs, sputum, exosomes)
• Fractionation (localization preserved)
Although Exosome structures are disrupted during fractionation,
membrane vs. lumen localization information is retained.
• Ultra trace quantification (TN-cyclon™)
• Dynamics analysis
Quantifying molecular changes associated with stimuli, treatment, or disease progression
—enabled by repeat sampling of real-specimens.
• Mechanistic interpretation
■Representative Research Example
Cancer x Exosomes (GRP78)
• GRP78 in the exosome lumen increases with stage progression. • After anti-cancer drug treatment, GRP78-rich exosomes are horizontally transmitted to surrounding cells. • Induce cancer stem cell formation and angiogenesis.
→ This will lead to elucidation of the mechanism behind the acquisition of treatment resistance
Figure: Example of dynamic analysis
https://doi.org/10.1016/j.ab.2022.114831
In this way, the ability to track molecular dynamics on an actual sample basis is a major feature of this service.
*Other research cases, such as infectious diseases and metabolism, can be viewed in the PDF documents at the bottom of the page.
■ Typical cases where BioPhenoMA is chosen
The following chart summarizes typical inquiries received from researchers.
■ Flow of contract research services
1. Research content interview (NDA)
2. Protocol review and quotation
3. Pretreatment, fractionation, and measurement system construction
4. Sample measurement (ultra-trace quantitative)
5. Dynamic analysis and mechanism interpretation
6. Report submission
* Requests can be made at any stage. We will respond flexibly.
[Download materials] :
• → Introduction to the protein dynamics analysis platform that quantifies real sample dynamics (PDF)
• → Brochure (PDF)
【inquiry】:
For inquiries regarding real sample dynamics analysis, please click here .